how to run automatically script on blender

hello,
i have my file.blend which contain a script python.
i want to run automatically ma script when i run my file.blend.
what can i do please?

Thank you, i already create a script with Qt interface and i make auto-run like your response.
But when i run my file.blend, the interface run automatically on front ground and not blender interface(Picture)



what can i do in this case please?

You mean, you want to see you QT Frame, and the “normal blender interface” instead of “nothing” ?