render broken

Thats strange i ve been working on ascene with ao subsurface scatering and ray transp. i ve done some test render and everything get fine. but now i try to render an animation and no more of this effect appears. no more ao no more subsurface scatering and no more ray transp. eveything is still enable but… is there something that activate or desactivate that kind of stuf that i may be activated

i find for ao and ray transp( ray tracing was disable)
i post the scene i hope to get some help.
http://rs207.rapidshare.com/files/55703097/fleur4.blend
click on free account then type the code and download
thanks