TileMap from Blender

Is it possible to generate a tilemap with Blender and python?
I have been exporting the animations from Blender as images and then join them all with external software.
The purpose of it is to generate a tilemap to be used for gamming programming inside Excel (VBA with win32 GDI API’s) for my students.

Yes. May I suggest you look to Katsbits for directions, that sight is dedicated to this sort of thing.